Insertion junction: LMJ.RY0402.101948_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.101948
Chromosome:chromosome 1
Location:1501180
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g007737 FBT1 Folate/pteridine transporter 1; (1 of 1) IPR004324//IPR011701//IPR020846 - Folate-biopterin transporter // Major facilitator superfamily // Major facilitator superfamily domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTTCAAGTCCTGGAACAGGCTCACGACCG
Internal bar code:ACGAGAACTATACATATCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:614
LEAP-Seq percent confirming:99.4675
LEAP-Seq n confirming:934
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TTCAGTCCAGCGCCTAAGTT
Suggested primer 2:CTCGCATAGCACTGGATGAA