| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.101948 |
| Chromosome: | chromosome 1 |
| Location: | 1501180 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007737 | FBT1 | Folate/pteridine transporter 1; (1 of 1) IPR004324//IPR011701//IPR020846 - Folate-biopterin transporter // Major facilitator superfamily // Major facilitator superfamily domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGAAGGTCATGGGGCTGGACCCCTCAC |
| Internal bar code: | TTCTTGCCGGTCGCCCTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 99.4697 |
| LEAP-Seq n confirming: | 5252 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGTCCAGCGCCTAAGTT |
| Suggested primer 2: | CTCGCATAGCACTGGATGAA |