Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.101990 |
Chromosome: | chromosome 7 |
Location: | 5215455 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g348750 | (1 of 2) PTHR10794:SF58 - CAAX AMINO TERMINAL PROTEASE FAMILY PROTEIN | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCATGAAGGGATGCAGATGAGGCGCGG |
Internal bar code: | GACGGAGGGTGTATCAACCGCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 700 |
LEAP-Seq percent confirming: | 98.3621 |
LEAP-Seq n confirming: | 2162 |
LEAP-Seq n nonconfirming: | 36 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAATCCTCCGAAACACAGG |
Suggested primer 2: | GGCTCTCAAAACTCACTCCG |