| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102003 |
| Chromosome: | chromosome 16 |
| Location: | 6434368 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673553 | (1 of 1) PTHR10992//PTHR10992:SF906 - ALPHA/BETA HYDROLASE FOLD-CONTAINING PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTAGTGCGGTCTGCCCAGCACGGTAGGTG |
| Internal bar code: | GTCCTCATCGGTCAGGGCAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 616 |
| LEAP-Seq percent confirming: | 99.4236 |
| LEAP-Seq n confirming: | 345 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTTGAAGCTGTGCTGAG |
| Suggested primer 2: | ACTGTACGGCTACGACCCAC |