Insertion junction: LMJ.RY0402.102060_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.102060
Chromosome:chromosome 2
Location:3658246
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g095151 (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACGATTTAAAGCCCTGCGCCGCGCCCGC
Internal bar code:TGTGCTGAGGAGTCCCCGTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:539
LEAP-Seq percent confirming:64.3204
LEAP-Seq n confirming:795
LEAP-Seq n nonconfirming:441
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCATCCACCCACCTAAC
Suggested primer 2:CACCCATTGCACTGACAAAC