Insertion junction: LMJ.RY0402.102063_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.102063
Chromosome:chromosome 2
Location:3623651
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095142 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGACCAAACAAGTATTGAATCTTGTAACT
Internal bar code:CGTGGTCTGGCCTGTGGGTCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:259
LEAP-Seq percent confirming:95.966
LEAP-Seq n confirming:904
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACCATCATCTGTCGTGTCC
Suggested primer 2:GCATGTTTGTGCGTACTGCT