| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102072 |
| Chromosome: | chromosome_1 |
| Location: | 2647306 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g015451 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGCTGGTGTTGAAGTCGAGGGGTCGGAAG |
| Internal bar code: | GAGACGGGTTAAGAGAGGAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 99.0942 |
| LEAP-Seq n confirming: | 8533 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGACTTTGAATTCGCTA |
| Suggested primer 2: | ACGGCTGAATTGAGGTGTTC |