| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102134 |
| Chromosome: | chromosome 16 |
| Location: | 2679505 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662200 | CYA14 | Putative adenylate cyclase; (1 of 1) 3.6.1.25//3.6.1.3 - Triphosphatase // Adenosinetriphosphatase / Triphosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGGGTTGGGTGCGAGTTCAGTAGTCAGG |
| Internal bar code: | GGCATAATTAAATCTCGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 317 |
| LEAP-Seq percent confirming: | 99.8125 |
| LEAP-Seq n confirming: | 5324 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTTTAGGTGGGCTGTGT |
| Suggested primer 2: | AAACCACCAGTCTCACCGTC |