| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102171 |
| Chromosome: | chromosome 16 |
| Location: | 2020547 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656950 | (1 of 1) PTHR13140:SF300 - PROTEIN TAG-278-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTAACGAAGAAACGATGTTGTTGAAGC |
| Internal bar code: | TTAGGTAAATATGCCAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1050 |
| LEAP-Seq percent confirming: | 99.9095 |
| LEAP-Seq n confirming: | 1104 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTTGTTCCACACATCCG |
| Suggested primer 2: | GTGAGGAAGGGTGTCGTGTT |