| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102178 |
| Chromosome: | chromosome 3 |
| Location: | 869507 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146587 | (1 of 3) PTHR24193//PTHR24193:SF87 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTACACGCAGCTGACACGTTCTGACAAA |
| Internal bar code: | CGTTCATTTGGTTTCCCTCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 395 |
| LEAP-Seq percent confirming: | 98.8411 |
| LEAP-Seq n confirming: | 597 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTCTCCTCCTACGCCTTG |
| Suggested primer 2: | GACCGTTGAAGGCTTAGCTG |