| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102183 |
| Chromosome: | chromosome 5 |
| Location: | 3054303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238800 | (1 of 6) 3.6.4.1 - Myosin ATPase / Actomyosin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTTTGATTGTGCTTGGACTTGACTGAT |
| Internal bar code: | GAGCACTGTATAGCAGTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 937 |
| LEAP-Seq percent confirming: | 99.554 |
| LEAP-Seq n confirming: | 2009 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGGTTAGAACGCCCTTT |
| Suggested primer 2: | GGAGGAACAGCTGCAGACA |