| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102191 |
| Chromosome: | chromosome 10 |
| Location: | 6058953 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463300 | (1 of 1) PTHR31515:SF0 - TRANSMEMBRANE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAATTGGCTCAGGGAATGGGGGAAGGGAA |
| Internal bar code: | CTCGCACCGATCGTCGGTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 99.7941 |
| LEAP-Seq n confirming: | 3877 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCAAAAGAAAGACAGG |
| Suggested primer 2: | CGCGTAATGTTTTCCATGTG |