| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102200 |
| Chromosome: | chromosome 5 |
| Location: | 1834354 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g242000 | CHLD,CHLD1 | (1 of 1) K03404 - magnesium chelatase subunit D (chlD, bchD); Magnesium chelatase subunit D, chloroplast precursor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAGAGTGCCATCGTGCAATGCTGAATAC |
| Internal bar code: | TTCGAGTTTGAGTGTCCTACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 99.0945 |
| LEAP-Seq n confirming: | 2736 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCAGCGTTTATGGAAGTG |
| Suggested primer 2: | CTCACTGTCTGGCTTGTCCA |