| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102202 |
| Chromosome: | chromosome 2 |
| Location: | 4885441 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103400 | OPR118 | OctotricoPeptide Repeat protein 118 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACCGCAACCTGTCACCGCCATCTTTGT |
| Internal bar code: | AGAGGGTAGTTTCTTTGTTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 99.1645 |
| LEAP-Seq n confirming: | 22432 |
| LEAP-Seq n nonconfirming: | 189 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGCTGCTACTGCTACTG |
| Suggested primer 2: | CTCTTCCTCCTCCTCCTCGT |