| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.102234 |
| Chromosome: | chromosome 3 |
| Location: | 637350 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145767 | RRP6,RRP6a | putative exosome subunit; (1 of 1) K12951 - cation-transporting P-type ATPase D (ctpD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCCTGAATCGTGTCATACAACCTCTGCA |
| Internal bar code: | ATGCCGGGATGTTATGCCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 55 |
| LEAP-Seq percent confirming: | 95.7105 |
| LEAP-Seq n confirming: | 714 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGAGGGAGAGGATGAGC |
| Suggested primer 2: | CAACAATTCTGGCAAGCTCA |