| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.102237 |
| Chromosome: | chromosome 9 |
| Location: | 5183399 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399215 | FAP341 | Flagellar Associated Protein 341; (1 of 140) IPR002048 - EF-hand domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGCCGGCCCGGCATGGCACATCCCTGC |
| Internal bar code: | GTCTTTGTGCAGAAGCCCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 98.0488 |
| LEAP-Seq n confirming: | 603 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCGTGTTGTCTGTCGT |
| Suggested primer 2: | CCCCAAGAATCAAGGAGTCA |