| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102246 |
| Chromosome: | chromosome 16 |
| Location: | 6012734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676400 | HEL63 | (1 of 1) K12811 - ATP-dependent RNA helicase DDX46/PRP5 (DDX46, PRP5); DEAD/DEAH box helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACAGGTGAGCCGTCCCACTATAGGACA |
| Internal bar code: | GTGCCCTCCATTAGCCTAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 103 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGTGACGCAAGTACCTAT |
| Suggested primer 2: | CTAGGACGGAGCTGGACTTG |