| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102292 |
| Chromosome: | chromosome 6 |
| Location: | 7965807 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g303650 | (1 of 1) K15027 - translation initiation factor 2D (EIF2D) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCTGCAAGAACTTGGACATCTTCTTAT |
| Internal bar code: | CGACGTCATAAATAATTTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 486 |
| LEAP-Seq percent confirming: | 90.1832 |
| LEAP-Seq n confirming: | 2756 |
| LEAP-Seq n nonconfirming: | 300 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTGGAAGGGTGTCACTG |
| Suggested primer 2: | CACACCTGACCCTCACCTTT |