Insertion junction: LMJ.RY0402.102311_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.102311
Chromosome:chromosome 16
Location:284123
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g694201 (1 of 1) PTHR12461//PTHR12461:SF46 - HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED // SUBFAMILY NOT NAMED; Cupin- Jumonji-like protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGGTTATTGCGCGCGAGGTCAGAAAGTA
Internal bar code:TGCGGGGATAGTACGGTTCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1184
LEAP-Seq percent confirming:99.2694
LEAP-Seq n confirming:10191
LEAP-Seq n nonconfirming:75
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TTATCACAGAGCAACGCGAC
Suggested primer 2:CACACCCAAATACACCCACA