Insertion junction: LMJ.RY0402.102312_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.102312
Chromosome:chromosome 6
Location:7214936
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g297900 (1 of 4) PTHR11266//PTHR11266:SF42 - PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17 // SUBFAMILY NOT NAMED 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACCTTGCCGTTGGCCACCACGACTCGCTC
Internal bar code:AAAAAATTACAGTGGCGGAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:516
LEAP-Seq percent confirming:98.3485
LEAP-Seq n confirming:1191
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTACCCTAGTGGAGGGCA
Suggested primer 2:CCTCCTGAGTTGCTGTCTCC