| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102320 |
| Chromosome: | chromosome 6 |
| Location: | 1588058 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g260550 | CYG1 | (1 of 70) 4.6.1.2 - Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTCGGTCGCACATGACACTTTCCGGTTC |
| Internal bar code: | CAGCTTATCAGTAGGTACTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1141 |
| LEAP-Seq percent confirming: | 99.5177 |
| LEAP-Seq n confirming: | 619 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAACTATCTGCTTCACG |
| Suggested primer 2: | CTTCACGTGCAAGGAGACAA |