| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102348 |
| Chromosome: | chromosome 16 |
| Location: | 6362759 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674000 | WDR2,LWD1 | (1 of 1) K11805 - WD repeat-containing protein 68 (WDR68, HAN11); WD-4 repeat family protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGCAACCGCGTCGTTGCCGGCGGGGGT |
| Internal bar code: | TCGCAGAGACGACTCGTGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 108 |
| LEAP-Seq percent confirming: | 76.8501 |
| LEAP-Seq n confirming: | 405 |
| LEAP-Seq n nonconfirming: | 122 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGGTCACTGCTTAACAA |
| Suggested primer 2: | ACTGGGAATGTCGGAGTTTG |