| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102369 |
| Chromosome: | chromosome 16 |
| Location: | 7714242 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691215 | (1 of 1) PF05603 - Protein of unknown function (DUF775) (DUF775) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGATGCAGGTACGACGTATGCCCCGGGG |
| Internal bar code: | GGGGCAGGACACCTGCTGCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 606 |
| LEAP-Seq percent confirming: | 96.4778 |
| LEAP-Seq n confirming: | 3150 |
| LEAP-Seq n nonconfirming: | 115 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTTAGCGACTCCAGCACT |
| Suggested primer 2: | GCCTCGGTCATTCCAAACTA |