Insertion junction: LMJ.RY0402.102375_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.102375
Chromosome:chromosome 10
Location:2490941
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g436200 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCGGCGCGCCCGGACCCGACGACGACG
Internal bar code:TGGGCGCTGGCTCCTTGGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:51
LEAP-Seq percent confirming:77.5862
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTCCCTGAACTTTTCCA
Suggested primer 2:CCTGAACCACTACAGCAGCA