| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102451 |
| Chromosome: | chromosome 11 |
| Location: | 454104 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467590 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGTCACGTCAAAAAACGCCGTGACCGAC |
| Internal bar code: | TACACCCTCGCGCTTGTATGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 98.7385 |
| LEAP-Seq n confirming: | 2035 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACGGCAGAAGAAGATGC |
| Suggested primer 2: | GTGCCTGTGCGTGATACTTG |