| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102459 |
| Chromosome: | chromosome 10 |
| Location: | 6342219 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465400 | (1 of 1) IPR026305 - Negative elongation factor A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTAGGGTGCCCGCGGACTAAGATTGAC |
| Internal bar code: | CCGAGGGCCGGAGGGGGGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 648 |
| LEAP-Seq percent confirming: | 99.3709 |
| LEAP-Seq n confirming: | 5213 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGTGTGTCTCCGCACTT |
| Suggested primer 2: | ACACACACACACACACACGC |