Insertion junction: LMJ.RY0402.102483_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.102483
Chromosome:chromosome 1
Location:3324636
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g020918 PREP1 (1 of 1) PTHR11851:SF68 - PRESEQUENCE PROTEASE 1, CHLOROPLASTIC/MITOCHONDRIAL-RELATED; Presequence protease 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATAAAAACACCCCACGGCGCACGCTCCCTC
Internal bar code:TACTACGGTATGGTCCACGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:461
LEAP-Seq percent confirming:96.3441
LEAP-Seq n confirming:896
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGTCCAGCTCGTAGTGC
Suggested primer 2:GCCCCACTGACTACACCCTA