| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102494 |
| Chromosome: | chromosome 3 |
| Location: | 628475 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145727 | (1 of 1) PTHR11946:SF53 - TYROSINE-TRNA LIGASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGATGCCTTTTGCCATTCATCAGGACTG |
| Internal bar code: | GGATAAATACGCCATCCCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 98.5704 |
| LEAP-Seq n confirming: | 2689 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGACCACTTTGAGAACA |
| Suggested primer 2: | TGTGCTGTGGTAAGAAAGCG |