Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.102497 |
Chromosome: | chromosome_10 |
Location: | 2319690 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g434800 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGCGCCTGAACGCTGCGCGCGGAAATCGG |
Internal bar code: | AGAGATGGAGGTGCACTGCAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 689 |
LEAP-Seq percent confirming: | 89.0649 |
LEAP-Seq n confirming: | 1181 |
LEAP-Seq n nonconfirming: | 145 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTAGTGACCGGGACAAGGAA |
Suggested primer 2: | GCCACGGTCAACTACCTCAT |