| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.102501 |
| Chromosome: | chromosome 12 |
| Location: | 523597 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493000 | (1 of 1) IPR006153//IPR020846 - Cation/H+ exchanger // Major facilitator superfamily domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGAGAACTAATGCTGCACGGAGCAAG |
| Internal bar code: | TGGTCCCTAATTTCGGCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 617 |
| LEAP-Seq percent confirming: | 98.6251 |
| LEAP-Seq n confirming: | 1076 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCTTCCCTCGTCTAGGA |
| Suggested primer 2: | TCCAGGAGTGTTTCAGGACC |