| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102501 |
| Chromosome: | chromosome 12 |
| Location: | 523601 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g493000 | (1 of 1) IPR006153//IPR020846 - Cation/H+ exchanger // Major facilitator superfamily domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATATACATGTTGACTACGCTTTCTTCA |
| Internal bar code: | GCGCGGCCAGAAGCCTGCCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 373 |
| LEAP-Seq percent confirming: | 99.7187 |
| LEAP-Seq n confirming: | 10635 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCCTTCCCTCGTCTAGGA |
| Suggested primer 2: | TCCAGGAGTGTTTCAGGACC |