| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102502 |
| Chromosome: | chromosome 9 |
| Location: | 923725 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401050 | (1 of 12) 2.7.11.18 - [Myosin light-chain] kinase / Smooth-muscle-myosin-light-chain kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGTGGCCCGGACCACCTTCGGTTTCAAC |
| Internal bar code: | GGACGACACCTAGCGACGACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 98.9023 |
| LEAP-Seq n confirming: | 7929 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGACCCGACCTCTTGTTAC |
| Suggested primer 2: | TATATGTGAACGCGGGTGAA |