| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102524 |
| Chromosome: | chromosome 3 |
| Location: | 7751734 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203451 | CYA5 | Adenylate cyclase; (1 of 70) 4.6.1.1 - Adenylate cyclase / ATP pyrophosphate-lyase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGAAAGCAGCAGTTGAATCATGGGACTC |
| Internal bar code: | GTGGAATCCGCACATATCTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 99.4169 |
| LEAP-Seq n confirming: | 682 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGAGTTAGTAGACGGCAG |
| Suggested primer 2: | GTCGTTGACGAGGAGGAAGA |