| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102551 |
| Chromosome: | chromosome 1 |
| Location: | 674851 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003532 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAACCCAATATGCGCAATCGGGGGGATG |
| Internal bar code: | GTCAGAAGGGAAGTCTCTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 99.5498 |
| LEAP-Seq n confirming: | 3980 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCCATGGCTTTGAGGTAT |
| Suggested primer 2: | ACGTTGAACCAATCCCGTAA |