| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102557 |
| Chromosome: | chromosome 16 |
| Location: | 775480 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g690750 | (1 of 18) PTHR10774 - EXTENDED SYNAPTOTAGMIN-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGCGCGAGTCCTGTTCACCTTCCTAGC |
| Internal bar code: | TGATGGGTTTGGGCATTAGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 78.8576 |
| LEAP-Seq n confirming: | 1850 |
| LEAP-Seq n nonconfirming: | 496 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGAGCTGTTCGATGACAA |
| Suggested primer 2: | AACACTATGCCTCACAGGGG |