| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102568 |
| Chromosome: | chromosome 9 |
| Location: | 7768271 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416309 | (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCATGCGGGCGGGGTTGCGTTGGCGGGT |
| Internal bar code: | TCGGACCAGCAGACTCTCCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 913 |
| LEAP-Seq percent confirming: | 99.6999 |
| LEAP-Seq n confirming: | 12293 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGACTAAGCCACCAGGAGC |
| Suggested primer 2: | CTCTTCGGTAAGTTCGCTGG |