Insertion junction: LMJ.RY0402.102583_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.102583
Chromosome:chromosome 9
Location:5516556
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g400478 DIV31 phosphatidylinositol 4-phosphate 5-kinase; (1 of 1) PTHR23086:SF8 - PROTEIN PPK-2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCGCTTAGTCCGCAGCAGGGGGCACTGT
Internal bar code:GGGCTTGGAGGAACACGAGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:623
LEAP-Seq percent confirming:97.4646
LEAP-Seq n confirming:8188
LEAP-Seq n nonconfirming:213
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:AGCATCAACAGCTTTGGCTT
Suggested primer 2:GAGAAGGAGGAGGAGGAGGA