| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102589 |
| Chromosome: | chromosome 5 |
| Location: | 1965412 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241638 | (1 of 1) IPR000253//IPR002562//IPR012337 - Forkhead-associated (FHA) domain // 3'-5' exonuclease domain // Ribonuclease H-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGGAGTGGCGGCCCGAGACAGTGGCAG |
| Internal bar code: | GGGTGTTATTGGCCCAGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 98.1538 |
| LEAP-Seq n confirming: | 319 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGTGGCAAGACGGAGAGG |
| Suggested primer 2: | ACTTCACGTCAACCACCTCC |