| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.102600 |
| Chromosome: | chromosome 17 |
| Location: | 2524659 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716400 | (1 of 1) K14816 - pre-60S factor REI1 (REI1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAGAGAAGAGCACCAGGGCACGGGGAG |
| Internal bar code: | GGGGACCACGAGAGTGAGCTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 628 |
| LEAP-Seq percent confirming: | 98.5048 |
| LEAP-Seq n confirming: | 1647 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGGTATTGTTTGGGCTGT |
| Suggested primer 2: | GCTCCACGAACCACTAGAGC |