| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102604 |
| Chromosome: | chromosome 3 |
| Location: | 8085515 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201050 | BSD2,ZNJ2 | (1 of 10) 5.3.4.1 - Protein disulfide-isomerase / S-S rearrangase; Bundle-sheath defective-2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAAGGTGCGCACTCCCGTGTTCCCTTGT |
| Internal bar code: | GCAATCTCCACTCATCGAGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1150 |
| LEAP-Seq percent confirming: | 99.8238 |
| LEAP-Seq n confirming: | 1700 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGAGCCTACCTCACCTG |
| Suggested primer 2: | CATCCTCGCTGAATTTCCAT |