| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102617 |
| Chromosome: | chromosome_9 |
| Location: | 281906 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392867 | FMG1-B | Flagella membrane glycoprotein, major form | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGTGTCGGCCACTTCCACCGCTGCCAACA |
| Internal bar code: | GGGCGGTACTGTACTCATCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1043 |
| LEAP-Seq percent confirming: | 99.593 |
| LEAP-Seq n confirming: | 5139 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTACAGGTTGGGGTCGAT |
| Suggested primer 2: | ACACCCTCCTGACCATCAAG |