| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.102626 |
| Chromosome: | chromosome 10 |
| Location: | 1916609 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432000 | DDB1 | (1 of 1) K10610 - DNA damage-binding protein 1 (DDB1); UV-damaged DNA binding complex subunit 1 protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCGTGTTCCGCCCACAGAGGCTGCCATC |
| Internal bar code: | GGTTTGGGGTGATTCGCGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 99.396 |
| LEAP-Seq n confirming: | 1152 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTTGGCGTTGACACATCA |
| Suggested primer 2: | CAAGAGGGGATAGCTTGCTG |