| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.102627 |
| Chromosome: | chromosome 3 |
| Location: | 8799075 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206033 | (1 of 1) PTHR23130//PTHR23130:SF94 - FAMILY NOT NAMED // PROTEIN F23B12.4, ISOFORM A | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTCTGAACAAGCGCCATTTGGAAGGGA |
| Internal bar code: | AGGGGCGGTAGCGTGTCCTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 98.6601 |
| LEAP-Seq n confirming: | 5375 |
| LEAP-Seq n nonconfirming: | 73 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | ACCCAGACCGACTCAAACAC |