| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102649 |
| Chromosome: | chromosome 16 |
| Location: | 5521184 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680500 | EFH10 | (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair; EF-hand Calcium binding protein, may be centrin-related | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAACGCATCAGCCGCGCTGCTGTCCCCA |
| Internal bar code: | GGTGCGGAAGTCTGGACGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 99.0711 |
| LEAP-Seq n confirming: | 2133 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAGTTGTGAAAGCGTGT |
| Suggested primer 2: | TGCAGGTCTAGCTGATGTGG |