| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102672 |
| Chromosome: | chromosome 5 |
| Location: | 1863681 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241750 | PDK3 | Pyruvate dehydrogenase kinase; (1 of 2) 2.7.11.2 - [Pyruvate dehydrogenase (acetyl-transferring)] kinase / Pyruvate dehydrogenase kinase (phosphorylating) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATTGGTTCAGCTCGGGTGCCGTACTGCA |
| Internal bar code: | GATCAAGAGTTAGTCGCGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 99.3827 |
| LEAP-Seq n confirming: | 161 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGGCTGGAACCAATACAG |
| Suggested primer 2: | GTACAGGCCCGATGAACACT |