| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.102676 |
| Chromosome: | chromosome 10 |
| Location: | 5230279 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457050 | (1 of 41) IPR000719//IPR011009 - Protein kinase domain // Protein kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTAACAGGCGCAGGGCTTGTGGCGCCCC |
| Internal bar code: | TTCTGGGCTTCACTGGAATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 448 |
| LEAP-Seq percent confirming: | 99.6885 |
| LEAP-Seq n confirming: | 2880 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCAAAGTGGGTAAGCCAA |
| Suggested primer 2: | TCTGTTAGTGGGCTCCTGCT |