| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102699 |
| Chromosome: | chromosome 2 |
| Location: | 2450241 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g091750 | (1 of 4) 2.7.9.5 - Phosphoglucan, water dikinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTGCCACCAGGACCTCCACAGCCGCAGG |
| Internal bar code: | TGGCTGACGAGCATATGCTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1029 |
| LEAP-Seq percent confirming: | 99.6422 |
| LEAP-Seq n confirming: | 15316 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGAAGAATGAGGGTGTT |
| Suggested primer 2: | AATGAACGACACCCTCGAAC |