| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102712 |
| Chromosome: | chromosome 12 |
| Location: | 7659558 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554450 | intron|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTACCGGCGCTGTGCGCTCAGTGCGCT |
| Internal bar code: | TGGAATCGGATCTCGGCTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 99.5536 |
| LEAP-Seq n confirming: | 2453 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGGAGCCAACATACACT |
| Suggested primer 2: | CCCAAACACGTAAGCCTCAT |