| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.102719 |
| Chromosome: | chromosome 3 |
| Location: | 8204418 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200000 | (1 of 2) PTHR10183//PTHR10183:SF323 - CALPAIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGTCCAGTAAGCCAGAGTTGTCTGCG |
| Internal bar code: | TGTTGGTCTATAACGGGCTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 684 |
| LEAP-Seq percent confirming: | 99.4007 |
| LEAP-Seq n confirming: | 2322 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACCTCAACAGCCATCACC |
| Suggested primer 2: | TTCTCCTGACGCAACATCTG |