| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.102730 |
| Chromosome: | chromosome 6 |
| Location: | 6233995 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290800 | RMG1 | RNA cap guanine-N2 methyltransferase; (1 of 1) 2.3.1.221 - Noranthrone synthase / Polyketide synthase A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTCAGATGCGGACAGGTGCCTGGCGTG |
| Internal bar code: | ACGTATCACTGGTTCCGTACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 558 |
| LEAP-Seq percent confirming: | 97.0899 |
| LEAP-Seq n confirming: | 4871 |
| LEAP-Seq n nonconfirming: | 146 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGGCATCACCCTGTATTG |
| Suggested primer 2: | GTTCTACTCGAGGCAGGACG |