Insertion junction: LMJ.RY0402.102730_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.102730
Chromosome:chromosome 6
Location:6233995
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g290800 RMG1 RNA cap guanine-N2 methyltransferase; (1 of 1) 2.3.1.221 - Noranthrone synthase / Polyketide synthase A 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTTCAGATGCGGACAGGTGCCTGGCGTG
Internal bar code:ACGTATCACTGGTTCCGTACGG

Confirmation - LEAP-Seq

LEAP-Seq distance:558
LEAP-Seq percent confirming:97.0899
LEAP-Seq n confirming:4871
LEAP-Seq n nonconfirming:146
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGGCATCACCCTGTATTG
Suggested primer 2:GTTCTACTCGAGGCAGGACG